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The Na-K-2Cl symporter requires that equal amounts of potassium and sodium be transported However, there is far less potassium in the lumen than sodium, and it seems that the lumen would be depleted of potassium long before very much sodium was reabsorbed Interestingly, the luminal membrane has a large number of potassium channels that allow much of the potassium transported into the cell on the Na-K-2Cl symporter to leak back (as described in 8, potassium recycles between the cytosol and lumen in order to be available for symport with sodium and chloride) Thus, under normal circumstances, luminal potassium does not limit sodium and chloride reabsorption through Na-K-2Cl symporters In addition to the active transcellular reabsorption of sodium, a large percentage (perhaps as much as 50%) of total sodium reabsorption in this segment occurs by paracellular diffusion There is a high paracellular conductance for sodium in the thick ascending limb, and the luminal potential in this segment is positive, a significant driving force for cations (We see in later chapters that this paracellular pathway also allows substantial reabsorption of potassium and calcium as well) However, none of this would work without the continuous operation of the Na-KATPase in the basolateral membrane To summarize the most important feature of the loop of Henle, the descending limb reabsorbs water but not sodium chloride, whereas the ascending limb reabsorbs sodium chloride but not water, with the net of the loop as a whole being reabsorption of more salt than water The ascending limb is called a diluting segment, and because Henle s loop as a whole has reabsorbed more solute than water, the fluid leaving the loop to enter the distal convoluted tubule is hypo-osmotic (more dilute) compared with plasma

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This code snippet is added to the CreateChildControls method and casts the Zone property of the Editor Part to a ToolPane object. This ToolPane object corresponds to the tool pane and exposes the Cancel button so you can turn off its validation.

Even though WINS servers are only used to support legacy applications, they are still required in most large networks Once a month, you should verify the proper operation of your WINS servers In most networks, there will be at least two WINS servers to provide redundancy for the service These servers should be replication partners using persistent connections

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Property dependencies If you re creating advanced Editor Parts, you might need to create one that adapts to the selections that the user is making. You can use standard ASP.NET postbacks to make the Editor Part react to the user s actions. Suppose you want to add caching to the RSS Web Part. Wouldn t it be great to have the caching and cache time configurable Just add two properties to the Web Part: one indicating if caching is on and another specifying the time to cache the data:

The major luminal entry step in the active reabsorption of sodium and chloride by the distal convoluted tubule is via the Na-Cl symporter (Figure 6 4) The characteristics of this transporter differ significantly from the TAL Na-K-2Cl symporter and are sensitive to different drugs In particular, the Na-Cl symporter is blocked by the thiazide diuretics, including hydrochlorthiazide (Sodium channels, like those in the collecting tubule principal cells, also exist in the distal convoluted tubule)3

[Personalizable(PersonalizationScope.Shared)] public bool CacheData { get; set; } [Personalizable(PersonalizationScope.Shared)] public int CacheTime { get; set; }

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To verify the status of your WINS servers, you need to perform three tasks: Check server statistics Scavenge the database to remove stale records Check WINS logs for errors You may also check database consistency and check for version ID consistency The latter deals with how WINS manages replication Each record is given a version ID The records with the highest version ID are replicated to the server s partners

Because you re going to use these properties in your Editor Part, you only need to specify the Personalizable attribute so that the values are stored correctly. To be able to edit these values, add a Check box and another TextBox to your Editor Part:

In the collecting ducts, there is a division of labor among several different cell types Reabsorption of sodium and water is associated with principal cells (so called because they make up approximately 70% of the cells; Figure 6 5) Principal cells also play a major role in maintaining potassium homeostasis (see 8) Reabsorption of chloride can occur partially via paracellular pathways, but active

You have to be a member of the local WINS Users group or the local Administrators group in order to operate and configure the WINS server

protected Check box m_cache; protected TextBox m_cacheTime;

Create these controls in the CreateChildControls method after the other controls. Listing 5.9 shows how the controls are created and added to the controls collection.

SECURITY SCAN To check server statistics: 1 Launch the Global MMC Console (Quick Launch Area | Global MMC Console) 2 Connect to the appropriate server (Action | Connect to another computer) and either type in the server name (\\servername) or use the Browse button to locate it Click OK when done 3 Move to the WINS service (Services and Applications | WINS) 4 Make sure you click the WINS service and that its information is displayed in the right pane, then right-click on WINS to select Display Server Statistics from the context menu 5 This will display current statistics for the server, including uptime, discovers, offers, requests, and more Make note of these values in your monthly WINS log Click Close when done You can use the same context menu to select Scavenge Database, Check Database Consistency, and Check Version ID Consistency

Besides sodium and chloride reabsorption, distal convoluted tubule cells are a major site for the control of calcium homeostasis because they have apical calcium channels that are regulated by parathyroid hormone (see 10)

this.Controls.Add(new Label(){ Text = "<br/>Cache data:<br/>", AssociatedControlID = "cache"}); m_cache = new Check box() { ID="cache" }; m_cache.CheckedChanged += new EventHandler(m_cache_CheckedChanged); m_cache.AutoPostBack = true; this.Controls.Add(m_cache); this.Controls.Add(new Label(){ Text = "<br/>Cache time:<br/>", AssociatedControlID="cacheTime"}); m_cacheTime = new TextBox() { ID = "cacheTime" }; this.Controls.Add(m_cacheTime);

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