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Epithelial transport requires that epithelial cells are polarized (ie, the proteins present in the apical and the basolateral membranes are not the same) This polarization can promote net flux of sodium from lumen to interstitium, which is the linchpin around which the transport of virtually every other substance depends Figure 4 4 shows the morphology of a generalized renal epithelium in which salt and water transport can be viewed as a 4-step process Step 1 is the active extrusion of sodium via Na-K-ATPase from the cell to the interstitium This creates a low concentration of sodium within the cell so that sodium moves downhill from the lumen to the cell interior via a variety of symporters, antiporters, and channels A key player in the proximal tubule is the sodium-proton antiporter (NHE-3 isoform) that we discuss further later The consequence of this transcellular sodium movement is the separation of charge (excess Na1 on the interstitial side) that promotes Step 2, the movement of anions through anion-specific transcellular and paracellular pathways to balance the positive charge The accumulation of sodium and anions in the interstitial space produces an osmotic gradient from lumen to interstitium that promotes water movement (Step 3) Finally, the accumulation of salt and water in the interstitium promotes the bulk flow of solute and water into the peritubular capillaries (Step 4) driven by Starling forces (see Table 4 1) In Step 1, any reabsorbed substance that enters the epithelial cells with sodium across the apical membrane must exit across the basolateral membrane The particular mechanism by which this occurs depends on the

Adding the button-click event manually Because you can t take advantage of the designer and automatically hook up the control events, you have to manually add the click event to the button. The event is wired up to the button control after the control is created in CreateChildControls. Using IntelliSense in Visual Studio helps you write the code. When you type += after the Button.Click event and press the Tab key, Visual Studio writes the code for you. If you press Tab a second time, it also creates the event handler method skeleton for you. The source code row you wrote should look like this:

3 To add keywords, click the Keywords button Type the keyword and click Add Click OK when done 4 Close the dialog box when done Repeat for each share you publish in AD

button.Click += new EventHandler(button_Click);

58 / 4

And the automatically created event handler looks like this:

2 2 2 2

void button_Click(object sender, EventArgs e) { throw new NotImplementedException(); }

Lumen Peritubular capillary Step 4 Various sodium permeability pathways Na+ Step 2 Anions Water Tight junctions Lateral space Interstitium

Replace the exception with the assignment of the text box text to the label just as we did in the Visual Web Part example. To complete your Web Part, your event handler code should look like this:

TIP Do not publish hidden shares (using the Share$ name format) because they will no longer be hidden Any share that is published in AD will be visible to users

void button_Click(object sender, EventArgs e) { label.Text = textBox.Text; }

2 2 2

As in the Visual Web Part example, you can change which base class the Web Part will use here but, because this sample is deployed to the sandbox, you can t use a base class that inherits from the SharePoint implementation. Doing so will result in a runtime error when you add the Web Part to a page, even though the compilation will work fine.

the peritubular capillary Everything depends on and follows logically from Step 1, which is the active extrusion of sodium into the interstitium This induces a parallel transport of anions (Step 2) The movement of sodium and anions generates an osmotic drive that causes reabsorption of water (Step 3) Finally, the increased volume in the interstitium alters peritubular Starling forces and induces the bulk flow of water and solute from interstitium into the peritubular capillary (Step 4)

Testing and debugging your Web Part To run your Web Part, press F5 (just like you did with the Visual Web Part), and SharePoint 2010 will open if the compilation succeeds. Any compilation or packaging errors will be reported and stop the debugging process. Once Internet Explorer has opened the SharePoint site, create a new page using the Site Actions menu. Or, you can edit the page you used earlier by clicking the Edit Page icon, inserting the Web Part on the page, and verifying that it s working. Because you just created a sandboxed solution, this Web Part is running in the isolated sandbox process. Visual Studio deployed it to a special gallery in the site collection called the Solution Gallery. Click Site Actions > Site Settings and then click Solutions under the Galleries header to access the Solutions Gallery. This gallery

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holds the WSP files that can be used within the site collection, and they re all running in the sandbox. Verify that your newly created solution is there. Visual Studio attaches the debugger to Internet Explorer and to the SPUCWorkerProcess.exe process (instead of the IIS Worker Process) that s the host for the sandbox.